Accuris qMAX™ One Step Kits allow for highly sensitive real time RT-qPCR assays to be performed directly from RNA templates. Workflows are simplified with optimized formulations of ready-to-use 2X qPCR master mix and 20X reverse transcriptase.
Optimized buffer includes powerful RNase inhibitors and an extremely thermostable MMLV-derived reverse transcriptase enables robust first strand cDNA synthesis. Accuris Hot Start Taq uses an antibody mediated hot start mechanism allowing for sample preparation at room temperature. Only after an initial incubation at 95C will the Taq become active, so non-specific amplification is greatly reduced. An inert blue dye is included in the Taq master mix to help simplify pipetting and reduce errors.
qMAX Green One Step kits incorporate our proprietary intercalating dye which exhibits higher fluorescent and lower PCR inhibition than other popular dyes such as SYBR.
qMAX Probe One Step kits are optimized for use with popular TaqMan, Scorpions, and molecular beacon probes.
qMAX Probe One Step 4X RT-qPCR Mix is a high-concentration, one-step reverse transcription quantitative PCR system optimized for sensitive detection of viral RNA and DNA targets. The system comprises a 4× qMAX™ Probe Master Mix and a separate 20× qMAX™ Reverse Transcriptase supplemented with an RNase inhibitor, enabling modular assay design and improved reagent utilization. The 4× master mix format permits increased template input per reaction, enhancing analytical sensitivity for low-copy targets. Additionally, the elevated concentration supports reduced total reaction volumes, facilitating rapid thermal cycling while maintaining amplification efficiency and reproducibility.
The formulation is specifically optimized for dual-labeled hydrolysis probe-based assays and is compatible with both standard and fast cycling protocols. It supports robust performance in Singleplex and multiplex RT-qPCR applications, including higher-order multiplexing (>3 targets). Buffer composition and enzyme system are engineered to maintain amplification efficiency across a broad range of template complexities, including GC-rich and AT-rich regions, while exhibiting tolerance to common PCR inhibitors. To maximize compatibility with multiplex assay designs and diverse instrumentation, the master mix is supplied without ROX passive reference dye, allowing user-defined normalization strategies where required.
qMAX Probe One Step Multiplex kits are specifically developed and optimized for efficient probe-based detection of multiple targets in a single reaction well. The Multiplex formulation is comprised of a 20x reverse transcriptase and 2x PCR Mix preparation ideally suited for complex RNA samples including low-copy number viral RNA commonly used in the clinical and research laboratory. qMAX Probe One Step Multiplex kits have been designed to overcome the many challenges of multiplex RT-PCR, by addressing important factors such as the balance between magnesium chloride and deoxynucleotide concentrations, the relative Taq Polymerase and reverse transcriptase concentration, and the ionic conditions of the core reaction buffer.
All Accuris One-Step Kits are compatible with standard and fast cycling protocols and provide increased sensitivity, speed and reproducibility for a broad range of samples and targets. The polymerase mix is available with different levels of ROX reference dye for compatibility with all qPCR instruments.
Bulk Packaging Available
To accommodate the requirements of high throughput users, Accuris is now offering their full line of qPCR and RT-qPCR reagents in bulk packaging at highly discounted prices. Bottle sizes of 25ml (2500 reactions) and 50ml (5000 reactions) are available for qMAX SYBR Green and qMAX Probe mixes. One Step RT-qPCR kits are also available in bulk format for 2500 and 5000 reactions and are ideal for labs performing pathogen detection.
