The Accuris SmartReader™ Multimode is an advanced microplate reader designed to meet the growing needs of advanced life science labs. Equipped with a full suite of high-performance optical systems, the SmartReader Multimode is your all-in-one solution for absorbance, fluorescence and luminescence applications.
The SmartReader Multimode quickly processes assays in standard 96 well plates and can also accept 6, 12, 24, 48 and 384 well plates. Programming and operation are managed from the instrument’s large color touch screen and no external computer or software is required. Optional PC software is available for easy control and data handling via a Windows™ PC. The friendly and intuitive interface guides the user through various options for assay set up and processing. The instrument features a built-in plate shaker with linear, orbital, and double-orbital shaking modes, and an incubator that can perform top heating from ambient + 4°C – 45°C. Top heating creates a temperature gradient to prevent condesnation when lids are used on the microplates. Upon startup, the SmartReader Multimode performs a self-diagnostic test, followed by an automatic calibration of the entire system, ensuring consistent results across a wide range of assays.
Utilizing an advanced monochromator and a long-life, broad-spectrum xenon flash lamp, the SmartReader Multimode can produce incident light at user-selected wavelengths from 200 to 1000 nm in 1 nm steps. Processing assays is fast; a full 96 well plate can be read in under 15 seconds. The kinetic assay feature performs multiple readings over a set period of time, with curves for each well displayed in real-time. The optional SmartDrop™ plate allows fast and convenient UV absorbance evaluation of up to 16 nucleic acid and protein sample droplets.
Three filter modules are included to provide the most common excitation and emission wavelengths needed for fluorescence assays. Additional filter modules are available. These advanced modules incorporate dichroic mirrors with narrow band-pass optical filters and ensure high light transmission, increased detection sensitivity, and are easily exchanged. Modules are barcoded for automatic loading of wavelength data. The SmartReader Multimode allows for automatic dynamic range selection, which allows for the gain of the photomultiplier tube to accurately capture and normalize the signal intensity of samples.
The SmartReader Multimode is capable of luminescent detection, and can process both flash and glow assays. An advanced PMT (photo multiplier tube) enhances sensitivity of weak signals, prevents over-saturation of strong signals, and provides an ideal detection range for all common luminescence applications. Up to 2 sample injectors (available separately) can be installed and are compatible with both 96 and 384-well plates.
Data from the SmartReader Multimode to can be exported as either a .XLS or .CSV file via the included USB flash drive, the FTP server, or by using the optional SmartReader™ PC Software.
The SmartReader PC Software allows for remote programming & operation of the instrument via cable connection. Data from the instrument is automatically uploaded to the connected PC for further analyses. Both a Research version for academic use, and a FDA 21 CFR Part 11 compliant version are available.
Model |
Accuris SmartReader™ Multimode |
| Measurement Technologies | Absorbance, Fluorescence, Luminescence |
| Plate Formats | 6- to 384-well plates |
| Shaking | Linear (4.7 Hz, 7.0 Hz, 13.5 Hz), orbital and double orbital (240 – 1200 rpm) |
| Incubation Range | RT +4 °C to 45 °C |
| Display | 10.0 inch touchscreen |
| Communication Ports | 2 USB A type ports / 1 USB B type port
1 Ethernet port RS232 interface |
| Dimensions (WxDxH) | 16.5 x 21.7 x 15.2 in.
(42.0 x 55.0 x 38.6 cm) |
| Power Input | AC 100 to 240 V, 50 to 60 Hz |
| Weight | 33 kg / 72.8 lbs |
Absorbance |
|
| Light Source | High Energy Xenon Flash Lamp |
| Detector | Photodiode |
| Wavelength Accuracy | 2 nm |
| Wavelength Repeatability | 0.2 nm |
| Bandwidth (FWHM) | > 2.5 nm |
| Wavelength Range | 200 – 1000 nm, 1nm steps |
| Absorbance | 0.0 – 4.0 OD |
| Resolution | 0.0001 OD |
| Accuracy | 96-precision mode:± (1.0 %+0.003Abs)@ (0.0-2.0Abs] ;± 2.0 % @ (2.0-3.0Abs] |
| Repeatability | CV < 1.0 % or SD< 0.003 Fast(0.0-3.0Abs]CV < 0.5 % or SD< 0.003 Accurate(0.0-3.0Abs] |
| Stray Light | 0.1 % @ 220 nm |
| Linearity | R2 > 0.999 @ [0.0-3.0Abs] |
| Measurement Speed | < 15 seconds |
Fluorescence |
|
| Reading Mode | Top Reading |
| Excitation Light Source | High Energy Xenon Flash Lamp |
| Detector | Photo Multiplier Tube (PMT) |
| Detection Limit | ≤ 1 pM |
| Wavelength Range | EX: 200 – 1000 nm; EM: 270 – 850 nm |
| Included Filter Cartridges | (EX / EM): 470 / 525nm ; 523 / 564nm ; 624 / 692nm |
| Linear Dynamic Range | 6 logs |
Luminescence |
|
| Detector | Photo Multiplier Tube (PMT) |
| Detection Limit | 15 amol / well |
| Linear Dynamic Range | 6 logs |
