Magnetic bead-based purification has become the preferred method for isolating DNA, RNA, and other target molecules from a variety of biological sample types. The quality and quantity of the extracted molecules can be significantly higher than other methods, such as spin columns, while the cost per sample can be lower. The IsoPure™ Mini from Accuris Instruments is a compact, automated purification system that improves efficiency and reproducibility using the magnetic bead-based method. Capable of processing up to 16 samples at one time, the instrument is easy to set up, program, and operate. Mixing, magnetic bead transfer, washing, and elution steps are performed automatically, saving valuable hands-on time. The purification process can be completed within 10 to 60 minutes depending on the type of reagent kit used and the complexity of the program.
Programming via Android™ App or PC Software
Creating a protocol is simple using the IsoPure app or PC software, both are included on a USB flash drive with each instrument. Mixing speed, sample temperature, and magnetization parameters can be all be programmed. When using the IsoPure app on an Android device, the saved protocols can be transferred to the instrument via Bluetooth connection or by using the included barcode scanner. The PC software can be used for programming and transferring by USB connection or barcode scanner. Free, lifetime App updates are available so users can access newly available features as they are introduced.
QR Code Scanning System
A handheld scanner is included with each IsoPure™ Mini, adding a convenient and fast method for loading a protocol onto the instrument. After creating or opening a protocol on a companion Android device or a connected PC, an option is available for displaying a QR code. Simply scan the QR code to instantly transfer the protocol. QR codes are also available in kit instruction manuals for instant loading of optimized programs.
Accuris Instruments has partnered with leading magnetic bead kit manufacturers to develop and optimize protocols for specific applications. To request scripts for specific kits, or for assistance in optimization, please contact the IsoPure™ Technical Support Team at firstname.lastname@example.org.
Sample Temperature Control
Heating sample mixtures during the lysis and elution steps can significantly improve results. Heating strips on the sample platform can be precisely set to any temperature between ambient +5°C to 120°C. Both deep well plates and 6-well reagent vessels fit into the contoured heating strips for efficient heat transfer. Two cooling fans maintain ambient temperature inside the housing and temperature of the heating strips.
The IsoPure™ Mini can store up to 100 protocol files in internal memory. These can be transferred between instruments or to a computer for storage or sharing. Simply insert the included flash drive to export the selected files. Internal protocol management can be done via the instrument touch screen, or by using the included mouse.
An ultraviolet sterilization lamp (UVC) inside the processing chamber provides effective elimination of most bacterial, viral and stray genomic DNA contaminants. This additional safeguard reduces the possibility of contamination and downstream amplification of unwanted targets.
Touch-Screen Control Panel
The control panel on top of the IsoPure™ Mini allows for quick selection of stored protocols and instrument control. To execute a protocol, select it from the file list, insert a prepared sample plate (or 6-well reagent vessel) and tip combs, and press the start button. Run parameters and operating status are displayed during sample processing.
Accuris offers magnetic tip combs, deep well plates, and 6-well sample strips designed to fit the IsoPure™ Mini. Produced using virgin polypropylene and packaged in a sterile environment, these consumables are guaranteed to be contaminant-free. The 8-place magnetic tip combs lock into position inside the instruments processing chamber. There are 2 options for sample vessels: 96 deep-well plates or 6-well sample strips. For processing up to 16 samples, deep well plates are used. When only 8 or fewer samples need to be processed, the 6-well sample strips are ideal, and these are loaded onto the included aluminum rack. These sample strips reduce waste and lower consumable costs when only a few samples need to be processed.
|Capacity/sample volume||16 samples / 50µl to 1000µl|
|Magnetic bead recovery||>95%|
|Lysis temperature range||Ambient +5⁰C to 120⁰C|
|Elution temperature range||Ambient +5⁰C to 120⁰C|
|Mixing Speed||Variable, 10 Levels|
|Programming Control||Bluetooth Android Device or PC (not included)|
|Program Memory||100 protocols (internal memory)|
|Communication Ports||USB for mouse, flash drive storage/transfer|
|Contamnation Control||UV lamp (3W, 253.7nm UVC wavelength)|
|Scannner||QR Code Scanner Included|
|Dimensions||7.9 x 10.2 x 11.8 in. (20 x 26 x 30 cm)|
|Weight||7.0 kg / 15.4 lbs|
|Electrical Input||Universal 120 to 240VAC|