For applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options. Our proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets.
Use our convenient PCR Selector Tool to determine the correct Accuris PCR Reagent for your applications.
For applications requiring highly accurate amplification, and full control of enzyme concentration, choose our High Fidelity DNA polymerase kit. Our polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples.
A 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length.
A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, Accuris High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.
The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.
Accuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, and an antibody mediated hot-start polymerase with proof-reading component for trouble-free PCR reaction assembly and performance.
The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.
Accuris AccuFi HFHS PCR Mix is optimized 2X master mix that includes AccuFi High Fidelity polymerase, a proprietary reaction buffer system, MgCl2, and ultra-pure dNTPs. The engineered polymerase exhibits rapid extension kinetics (~10 s/kb), enabling efficient fast cycling protocols while maintaining high processivity. The formulation supports robust long-range amplification, with reliable performance on templates exceeding 15 kb. The enzyme possesses both 5’→3’ DNA polymerase activity and intrinsic 3’→5’ exonuclease proofreading activity, resulting in ultra-low error rate (below the detection limit) compared to Taq DNA polymerase. The high fidelity of the enzyme supports applications where sequence integrity is critical, such as molecular cloning, next-generation sequencing (NGS) library construction, and site-directed mutagenesis. The optimized buffer system enhances amplification efficiency across challenging templates, including GC-rich and partially impure DNA samples, while maintaining high yield and specificity with minimal reaction optimization.
