| Model | Smart-Q™ 100 Fluorometer | Smart-Q™ 800 Fluorometer |
|---|---|---|
| Display | 7.0-inch, color touch screen | 7.0-inch, color touch screen |
| Dynamic Range | 5 orders of magnitude | 4 orders of magnitude |
| Light Source | Blue and Red LED | Blue and Red LED |
| Excitation Wavelengths | Blue: 460 ± 20nm and Red: 625 ± 20nm | Blue: 460 ± 20nm and Red: 625 ± 20nm |
| Emission Wavelengths | Green: 525 – 570nm and Red: 670 – 725nm | Green: 525 – 570nm and Red: 670 – 725nm |
| Stability | ≤ 1.5% | ≤ 1.5% |
| Detector | Photodiodes (300-1000nm) | Photodiodes (300-1000nm) |
| Measurement Speed | ≤ 5 seconds | ≤ 6 seconds |
| Consumable type | 0.5mL assay tubes | 0.2mL assay tubes (single and 8-tube strip) |
| Input Voltage | 24 VDC 2A | 24 VDC 2A |
| Dimensions (WxDxH) | 16.1 x 28.7 x 6.1cm / 6.34 x 11.30 x 2.40in. | 16.1 x 28.7 x 7.6 cm / 6.3 x 11.3 x 3.0 in. |
| Weight | 1.6kg / 3.5lbs | 2.0kg / 4.4lbs |
Smart-Q 100 for single sample processing
Smart-Q 800 for up to 8-sample processing
Accuris DNA Quantification Kits
The Accuris™ Smart-Q™ 100 & Smart-Q™ 800 Fluorometers are designed for the precise quantification of DNA, RNA, and protein samples. By utilizing well-established methodologies for fluorescence detection, the Smart-Q Fluorometers provide higher specificity and enhanced accuracy compared to the traditional method of UV-absorbance detection. Accurate results are particularly crucial in workflows involving:
The Smart-Q Fluorometers are engineered to achieve optimal performance with Accuris’ Fluorescent DNA Quantification Kits and assay tubes; however, they also maintain compatibility with a range of kits offered by various other suppliers.
Each instrument is equipped with a 7-inch color touchscreen, featuring an intuitive, icon-driven user interface. Preprogrammed protocols allow for quick & effortless assay set-up. Results are quickly calculated and displayed in less than 6 seconds, and saved to the instrument’s internal memory. All data can also be exported easily to a PC using a USB flash drive.
The Smart-Q Fluorometers, along with their accompanying assay kits, are engineered for the precise measurement of fluorescent dye signals that are bound to targeted biological molecules. These specially formulated dyes exhibit selective affinity towards DNA, RNA, or proteins, and generate fluorescent signals exclusively upon binding to their respective targets. Both Smart-Q models include Blue and Red channels that allow for various fluorescent dyes to be used in nucleic acid and protein measurements.
The Smart-Q Fluorometers incorporate advanced curve-fitting algorithms to establish calibration curves from standard samples of known concentrations.
The concentration of an unknown sample, whether it be DNA, RNA, or protein, is determined by correlating its Relative Fluorescence Units (RFUs) with those of the standards employed during calibration. The measurement sensitivity for each type of assay is distinct and defined by its detection limits.
The Smart-Q portfolio includes two distinct models, each designed to meet specific laboratory requirements:
The Smart-Q 100 allows for single-sample processing in 0.5mL assay tubes (NS1020-T). This renders the Smart-Q 100 ideal for laboratories processing fewer samples.
The Smart-Q 800 caters to high-throughput workflows and allows for up to eight samples to be evaluated at once using individual 0.2mL tubes or 8 x 0.2mL strips tubes (Order no. F1001-T and F1001-T-8, ). The use of a standard 8-channel pipettor can streamline both the reagent preparation and sample transfer, making the Smart-Q 800 an efficient solution for workflows requiring parallel processing of multiple samples.
Accuris DNA Quantification Kits are designed for accurate fluorescent quantification of dsDNA using an instrument such as the SmartDrop XF, Qubit™or Accuris™ Fluorometer.
Accuris 1X BR dsDNA Quantification Kit (Cat # F1000-BR1-100)
The Accuris 1X BR Double-Stranded DNA (dsDNA) Quantification Kit provides a highly sensitive, precise, and efficient method for quantifying dsDNA concentrations. This kit includes a ready-to-use assay working solution and dsDNA standards, ensuring both reproducibility and ease of application. Designed for optimal specificity, the assay selectively binds to dsDNA, detecting concentrations ranging from 2 to 1000 ng, thereby offering exceptional stability, an extended linear dynamic range, and enhanced sensitivity compared to conventional DNA quantification techniques.
Accuris BR dsDNA Quantification Kit (Cat # F1000-BR-100)
The Accuris dsDNA Broad Range (BR) Quantification kit makes DNA quantification easy and accurate. This assay kit is highly sensitive and selective for dsDNA due to the dye’s high quantum yield and large molar extinction coefficient — capable of detecting dsDNA between 2 and 1000 ng. The kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional broad -range dsDNA quantification kits.
Accuris 1X HS dsDNA Quantification Kit (Cat # F1000-HS1-100)
The Accuris 1X Double-Stranded DNA (dsDNA) Quantification Kit provides a simple, sensitive, and precise method for quantifying small amounts of dsDNA. This kit includes a ready-to-use assay working solution and dsDNA standards, ensuring both convenience and reliability. Engineered for high selectivity, the kit detects dsDNA concentrations ranging from 0.2 to 100 ng, offering exceptional stability, a broader linear range, and enhanced sensitivity compared to conventional DNA quantification kits.
Accuris HS dsDNA Quantification Kit (Cat # F1000-HS-100)
The Accuris HS Double-Stranded DNA (dsDNA) Quantification Kit simplifies DNA quantification while ensuring high accuracy. This high-sensitivity assay kit is specifically designed for dsDNA detection, leveraging a fluorescent dye with a high quantum
yield and large molar extinction coefficient for superior performance. With a detection range of 0.2 to 120 ng, the HS dsDNA Quantification Kit offers enhanced stability, a broader linear dynamic range, and greater sensitivity compared to traditional dsDNA quantification kits.
Accuris RNA BR Quantification Kit (Cat # F1000-RBR-100)
The Accuris RNA Broad Range (BR) Quantification Kit provides a simple, accurate, and highly selective method for RNA quantification. Designed for specificity to RNA over single-stranded (ssDNA) and double-stranded DNA (dsDNA) (see figure below), the kit enables reliable detection of RNA concentrations ranging from 1 ng/μL to 1000 ng/μL. This kit offers superior stability and sensitivity compared to other broad-range RNA quantification kits.
Accuris RNA HS Quantification Kit (Cat # F1000-RHS-100)
The Accuris RNA High Sensitivity (HS) Quantification Kit offers a simple, accurate, and highly sensitive method for RNA quantification. Designed with preferential selectivity for RNA over single-stranded (ssDNA) and double-stranded DNA (dsDNA) (see figure below), this kit reliably detects RNA concentrations ranging from 100 pg/μL to 100 ng/μL. Compared to conventional RNA quantification kits, the Accuris RNA HS Kit provides significant advantages in stability, linear dynamic range, and sensitivity.
Accuris ssDNA Quantification Kit (Cat # F1000-SD-100)
The Accuris ssDNA Quantification Kit provides an easy and accurate method for the quantification of single-stranded DNA (ssDNA) or oligonucleotides. The assay can detect ssDNA concentrations between 1 and 200 ng. This kit offers advantages in stability, linear dynamic range, and sensitivity over other traditional ssDNA quantification kits. Although
the Accuris ssDNA Quantification Kit is not specifically selective for ssDNA over double-stranded DNA or RNA, it is selective for short oligonucleotides. Furthermore, low concentrations of contaminants such as proteins, salts, solvents, and detergents do not interfere with assay sensitivity and accuracy.
